Enzymic polynucleotide synthesis primed by polyvinylalcohol linked oligothymidylate
نویسندگان
چکیده
منابع مشابه
Enzymic synthesis of N-acetyl-D-mannosamine.
As repor ted earlier 1, an e n z y m e purif ied f rom Clostridium per#ingens (N-ace ty lneuramin ic acid aldolase; NaNaldolase) ca ta lyzes t he following reversible reac t ion: p y r u v a t e + N-acetyl-Dm a n n o s a m i n e (N-AcMm) ~ N-ace t y l neu rami n i c acid (sialic acid). The recogni t ion of N A c M m as a na tu ra l l y occurr ing suga r leads to t h e ques t ion of t he m e c h...
متن کاملEnzymic Mechanism of Starch Synthesis in Plants
An important development in the field of polysaccharide biochemistry has been the discovery of starch synthetase in beans, potatoes and corn seedlings by Leloir and coworkers•>. The enzyme was found to be closely bound to the starch granules and to catalyze the transfer of glucose moiety from UDP-glucose into polysaccharides containing linkage of the a-D-(H4) type. Subsequently, the granule-bou...
متن کاملThe enzymic synthesis of ethanolamine plasmalogens.
Enzymic synthesis of ethanolamine plasmalogens from l-14C-hexadecanol in cell-free systems from Ehrlich ascites and Freputial gland tumors of mice is documented. Identiiication of the ethanolamine plasmalogens is based on the preparation and chromatographic behavior of a number of chemical derivatives that take into account all three positions on the glycerol moiety. 14C-Derivatives isolated fr...
متن کاملRNA-primed DNA synthesis in vitro.
In vitro DNA synthesis on single-stranded circular DNA can be initiated by RNA primers. RNA chains are covalently extended by DNA polymerase II from KB cells and DNA polymerase I from Micrococcus luteus, but not by an RNA-dependent DNA polymerase from avian myeloblastosis virus. The reaction product consists of DNA chains with a piece of RNA at their 5'-ends, hydrogen bonded to the template DNA...
متن کاملRemoval of low density lipoprotein from blood plasma using cross-linked, sulfated polyvinylalcohol.
The properties of a new synthetic LDL binding material, consisting of fragments of loosely crosslinked hydrogel, based on sulfated polyvinylalcohol are described. When incubated with 20 times its volume of plasma, this material binds up to 95% of the LDL, even from plasma with severely elevated LDL cholesterol levels (up to 20 mM). In addition a cholesterol-rich subfraction of VLDL is bound but...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1975
ISSN: 0014-5793
DOI: 10.1016/0014-5793(75)80474-1